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Image Search Results
Journal: Discover Oncology
Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
doi: 10.1007/s12672-025-03863-8
Figure Lengend Snippet: Enrichment of CSCs derived from endometrial carcinoma cells. A Representative images of spheres formed in serum-free medium on days 3, 6, 9, and 12. B , C Western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4) in parental cells (PCs) cultured in serum-supplemented medium and CSCs maintained in serum-free medium for 12 days. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
Article Snippet:
Techniques: Derivative Assay, Western Blot, Cell Culture
Journal: Discover Oncology
Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
doi: 10.1007/s12672-025-03863-8
Figure Lengend Snippet: The effects of Quercetin treatment on sphere formation and maintenance of stemness in CSCs. A 1 × 10 4 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and spheres were imaged. B 100 spheres with diameter > 50 μm were treated with 50 µmol/L Quercetin for 72 h, and then imaged. C 1 × 10 5 cells were treated with 25 or 50 µmol/L Quercetin for 12 days, and total protein was extracted for western blot analysis of stemness markers (ALDH1A1, c-Myc, Nanog, and Oct4). Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. All cytological experiments were independently repeated at least three times
Article Snippet:
Techniques: Western Blot
Journal: Discover Oncology
Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
doi: 10.1007/s12672-025-03863-8
Figure Lengend Snippet: Quercetin inhibits STAT3’s transcriptional activity in the presence of ERα. A Luciferase reporter assay was used to evaluate the inhibitory effect of Quercetin on STAT3-mediated Oct4 promoter activity. Different letters (a, b) indicate significant differences ( P < 0.05) between groups. B mRNA expression of Oct4, Nanog, Twist, and Snai1 was measured by qPCR after Quercetin treatment. Different letters (a, b, c) indicate significant differences ( P < 0.05) between groups. The effects of Quercetin on sphere formation ( C ) and invasion ( D ) were assessed in the presence or absence of ERα. All cytological experiments were independently repeated at least three times
Article Snippet:
Techniques: Activity Assay, Luciferase, Reporter Assay, Expressing